Differential expression



Figure 1. Differential expression of the guide RNAs for 1110034B05Rik across the contrasts of interest. Shown in logarithm of counts per million reads, without batch correction.



Figure 2. Differential expression of the guide RNAs for 1110034B05Rik across the contrasts of interest. Shown in logarithm of counts per million reads, corrected for batch



Table 1. Differential expression of the guide RNAs for 1110034B05Rik in logarithm of counts per million reads
Differential expression logCPM
IBET.D4.R1 IBET.D8.R1 IBET.D4.R2 IBET.D8.R2 IBET.D14.R1 IBET.D14.R2 Guide Batch
2.063802 9.048999 2.063802 9.779825 11.070178 10.41438 1110034B05Rik.10 Uncorrected
12.375037 10.509883 4.681162 9.779825 10.486618 11.41217 1110034B05Rik.3 Uncorrected
12.637882 11.788296 5.558541 11.774669 10.486618 12.41106 1110034B05Rik.4 Uncorrected
2.076307 9.061504 2.051297 9.767320 11.082683 10.40188 1110034B05Rik.10 Corrected
11.125307 9.260153 5.930892 11.029555 9.236888 12.66190 1110034B05Rik.3 Corrected
11.776462 10.926876 6.419961 12.636090 9.625198 13.27248 1110034B05Rik.4 Corrected




Gene level

Table 2. Competitive Gene Set Test for 1110034B05Rik
Camera
Contrast Gene nGuides Direction Pvalue FDR
IBET.D8-IBET.D4 1110034B05Rik 3 Down 0.5501135 0.9674330
IBET.D14-IBET.D4 1110034B05Rik 3 Up 0.9443553 0.9780702



Table 3. Gene level information 1110034B05Rik
Gene level
Contrast Gene nGuides Mean logFC IQR logFC Direction mean logFC Direction smallest Pvalue Stouffer’s Pvalue Stouffer’s FDR
IBET.D8-IBET.D4 1110034B05Rik 3 2.914561 5.381605 Up Down 0.6999572 0.9405675
IBET.D14-IBET.D4 1110034B05Rik 3 3.182062 5.987194 Up Down 0.0702977 0.3639801



Figure 3. Enrichment across guide RNAs of 1110034B05Rik. Each vertical line across the plot is a guide RNA.




Comparing contrasts

Table 4. Mean log fold-change for the guide RNAs of 1110034B05Rik
Mean logFC across contrasts
Gene IBET.D8-IBET.D4 IBET.D14-IBET.D4
1110034B05Rik 2.91456089840496 3.18206179565248



Figure 4. Boxplot comparing the guide RNAs for 1110034B05Rik across the contrasts of interest.